| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Treponema pallidum | AAGCAGCGAGTGTGCAGT | CGCGAAAGTAACCGTCAGC | 59.89 | 59.59 | 254 | 143 |
100.00%
|
100.00%
|
| Primer 2 | Treponema pallidum | TGCAAACACGCAGGAAACG | ACACACCCCAAGCAAGCA | 59.94 | 59.72 | 177 | 132 |
100.00%
|
100.00%
|
| Primer 3 | Treponema pallidum | TTGTGCGTGCATTGCGG | ACACACCCCAAGCAAGCA | 59.70 | 59.72 | 225 | 130 |
100.00%
|
100.00%
|
| Primer 4 | Treponema pallidum | CAAACACGCAGGAAACGCT | ACACACCCCAAGCAAGCA | 59.64 | 59.72 | 175 | 128 |
100.00%
|
100.00%
|
| Primer 5 | Treponema pallidum | GCGACAGCACACACCAAA | TTCTTTTCCACGCGTGCG | 58.89 | 59.37 | 252 | 124 |
100.00%
|
100.00%
|
| Primer 6 | Treponema pallidum | CGCAGCAGCAAAGCGTTT | ACGCGTAAGGCAACAGCT | 60.35 | 59.97 | 176 | 120 |
100.00%
|
100.00%
|
| Primer 7 | Treponema pallidum | AAGTGAAGAGAGCGCACCC | TTTGGCACACGTACCGGT | 60.00 | 59.49 | 169 | 117 |
100.00%
|
100.00%
|
| Primer 8 | Treponema pallidum | TTGGTTCGTGGAAGCGCT | ATTCCCAAACCGCACGCA | 59.89 | 60.60 | 178 | 117 |
100.00%
|
100.00%
|
| Primer 9 | Treponema pallidum | TTGGTTCGTGGAAGCGCT | TTCCCAAACCGCACGCAA | 59.89 | 60.83 | 177 | 117 |
100.00%
|
100.00%
|
| Primer 10 | Treponema pallidum | TTGGTTCGTGGAAGCGCT | GGCAATTCCCAAACCGCA | 59.89 | 58.94 | 182 | 117 |
100.00%
|
100.00%
|